Product Info Summary
SKU: | A10658 |
---|---|
Size: | 100μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-V-ATPase D1 ATP6V0D1 Antibody
SKU/Catalog Number
A10658
Size
100μl
Form
Liquid
Description
Boster Bio Anti-V-ATPase D1 ATP6V0D1 Antibody catalog # A10658. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-V-ATPase D1 ATP6V0D1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10658)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human V-ATPase D1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10658 is reactive to ATP6V0D1 in Human, Mouse, Rat
Applications
A10658 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
40.329kDa
Background of ATP6V0D1
Anti-CAND2 antibody is ideal for western blotting, ELISA and IHC. CAND2 is also known as TIP120B, and TATA-binding protein-interacting protein 120B. While both CAND1 (TIP120A) and CAND2 (TIP120B) are TATA-binding proteins and form complexes with various nuclear proteins involved in the control of eukaryotic gene transcription, CAND2 (TIP120B) is expressed specifically in muscle and heart tissue. This is contrary to the ubiquitous expression of CAND1 (TIP120A). TIP120 homologs exist in various higher eukaryotes including D. melanogaster, C. elegans, and A. thaliana. TIP120B is 60% identical in amino acid sequence to TIP120A.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of extracts from NIH-3T3, K562 cells, using V-ATPase D1 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For ATP6V0D1 (Source: Uniprot.org, NCBI)
Gene Name
ATP6V0D1
Full Name
V-type proton ATPase subunit d 1
Weight
40.329kDa
Superfamily
V-ATPase V0D/AC39 subunit family
Alternative Names
32 kDa accessory protein; ATP6D; ATP6DV; ATP6V0; ATPase, H+ transporting, lysosomal (vacuolar proton pump), member D; ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d isoform 1; ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d1; FLJ43534; H(+)-transporting two-sector ATPase, subunit D; P39; Vacuolar proton pump subunit d 1; V-ATPase 40 kDa accessory protein; V-ATPase AC39 subunit; V-ATPase, subunit D; VATX; Vma6; VPATPDV-ATPase subunit d 1; V-type proton ATPase subunit d 1 ATP6V0D1 ATP6D, ATP6DV, P39, VATX, VMA6, VPATPD ATPase H+ transporting V0 subunit d1 V-type proton ATPase subunit d 1|32 kDa accessory protein|ATPase, H+ transporting, lysosomal (vacuolar proton pump), member D|ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d1|H(+)-transporting two-sector ATPase, subunit D|V-ATPase 40 KDa accessory protein|V-ATPase AC39 subunit|V-ATPase subunit d 1|V-ATPase, subunit D|vacuolar proton pump subunit d 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATP6V0D1, check out the ATP6V0D1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATP6V0D1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-V-ATPase D1 ATP6V0D1 Antibody (A10658)
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No publications found for A10658
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-V-ATPase D1 ATP6V0D1 Antibody
Question
Do you have a BSA free version of anti-V-ATPase D1 antibody A10658 available?
K. Jones
Verified customer
Asked: 2020-04-10
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-V-ATPase D1 antibody A10658 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-10
Question
Is this A10658 anti-V-ATPase D1 antibody reactive to the isotypes of ATP6V0D1?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
The immunogen of A10658 anti-V-ATPase D1 antibody is Synthesized peptide derived from the Internal region of human V-ATPase D1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-03
Question
We are currently using anti-V-ATPase D1 antibody A10658 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
The anti-V-ATPase D1 antibody (A10658) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-13
Question
I was wanting to use your anti-V-ATPase D1 antibody for WB for mouse brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse brain identification?
E. Patel
Verified customer
Asked: 2018-04-30
Answer
You can see on the product datasheet, A10658 anti-V-ATPase D1 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-30
Question
Would anti-V-ATPase D1 antibody A10658 work on pig WB with osteoclastoma?
S. Jackson
Verified customer
Asked: 2015-02-26
Answer
Our lab technicians have not validated anti-V-ATPase D1 antibody A10658 on pig. You can run a BLAST between pig and the immunogen sequence of anti-V-ATPase D1 antibody A10658 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig osteoclastoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-02-26